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Data from: Aerosol transmission from infected swine to ferrets of an H3N2 virus collected from an agricultural fair and associated with human variant infections

Metadata Updated: November 10, 2020

A controlled influenza A virus transmission experiment was conducted in biosafety level 2 containment from January 23, 2018- February 13, 2018 assessing the respiratory droplet transmission efficiency of A(H3N2)/swine/Ohio/A01354299/2017 from experimentally inoculated pigs to naïve, contact ferrets. This experimental setting models the human-swine interface at agricultural fairs, where human infection with influenza viruses of swine origin has been well documented. Five pigs were housed in a standard enclosure and four ferrets were housed in isolators placed 7.5cm from the pig enclosure. Nasal swab samples from pigs were collected on 0, 1, 3, and 5 days post infection and nasal wash samples from ferrets were collected 0, 2, 4, 6, 8, and 15 days post contact. The whole genome sequences of 15 swine and 12 ferret virus isolates were obtained from respiratory samples cultured on Madin-Darby Canine Kidney cells. Sequencing was performed on an Illumina MiSeq platform and consensus sequences for each gene segment were generated using in-house software at the USDA-APHIS National Veterinary Services Laboratory.

Access & Use Information

Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Metadata Created Date November 10, 2020
Metadata Updated Date November 10, 2020

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 10, 2020
Metadata Updated Date November 10, 2020
Publisher Agricultural Research Service
Identifier a6b750cd-9554-4a66-81c3-2082a2c879fb
Data Last Modified 2020-06-16
Public Access Level public
Bureau Code 005:18
Metadata Context
Schema Version
Catalog Describedby
Harvest Object Id c4b675f6-8460-4965-8a8e-d2763ab16b99
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
Program Code 005:040
Source Datajson Identifier True
Source Hash 07cc48f95782bc8552d5718fbf14a5e537d4c2de
Source Schema Version 1.1

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