Culex quinquefasciatus Life Cycle, Development Stages Lab Experiment, Water Filter Sampling Data and Quantitative PCR Results, 2024-2025
These data consist of quantitative PCR results obtained from a targeted assay designed to detect the presence of Culex quinquefasciatus DNA shed into water. Under controlled laboratory conditions, water samples in three replicate container systems were collected during a laboratory experiment that spanned the larval developmental life stages of Culex quinquefasciatus from egg rafts through adult emergence, with sampling also encompassing the pre-egg raft experimental placement timepoint and beyond post-adult emergence (n=40 samples). The data also include negative control water samples taken alongside experiment samples (n=18) and blank samples taken as controls during the DNA extraction process (n=2).
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "010:12" ] |
| contactPoint |
{ "fn": "M. Renee Bellinger", "@type": "vcard:Contact", "hasEmail": "mailto:mbellinger@usgs.gov" } |
| description | These data consist of quantitative PCR results obtained from a targeted assay designed to detect the presence of Culex quinquefasciatus DNA shed into water. Under controlled laboratory conditions, water samples in three replicate container systems were collected during a laboratory experiment that spanned the larval developmental life stages of Culex quinquefasciatus from egg rafts through adult emergence, with sampling also encompassing the pre-egg raft experimental placement timepoint and beyond post-adult emergence (n=40 samples). The data also include negative control water samples taken alongside experiment samples (n=18) and blank samples taken as controls during the DNA extraction process (n=2). |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Digital Data", "format": "XML", "accessURL": "https://doi.org/10.5066/P13QHKMG", "mediaType": "application/http", "description": "Landing page for access to the data" }, { "@type": "dcat:Distribution", "title": "Original Metadata", "format": "XML", "mediaType": "text/xml", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.6881d3acd4be0232bdf2ae29.xml" } ] |
| identifier | http://datainventory.doi.gov/id/dataset/USGS_6881d3acd4be0232bdf2ae29 |
| keyword |
[ "Hawaii", "Hawaii Volcanoes National Park", "USGS:6881d3acd4be0232bdf2ae29", "biota", "environment", "environmental DNA", "field inventory and monitoring", "health", "mosquito larval development", "mosquito monitoring", "quantitative polymerase chain reaction", "water sampling" ] |
| modified | 2026-04-08T00:00:00Z |
| publisher |
{ "name": "U.S. Geological Survey", "@type": "org:Organization" } |
| spatial | -155.250614, 19.418334, -155.246429, 19.421187 |
| theme |
[ "geospatial" ] |
| title | Culex quinquefasciatus Life Cycle, Development Stages Lab Experiment, Water Filter Sampling Data and Quantitative PCR Results, 2024-2025 |