{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "M. Renee Bellinger", "hasEmail": "mailto:mbellinger@usgs.gov"}, "description": "These data consist of quantitative PCR results obtained from a targeted assay designed to detect the presence of Culex quinquefasciatus DNA shed into water. Under controlled laboratory conditions, water samples in three replicate container systems were collected during a laboratory experiment that spanned the larval developmental life stages of Culex quinquefasciatus from egg rafts through adult emergence, with sampling also encompassing the pre-egg raft experimental placement timepoint and beyond post-adult emergence (n=40 samples). The data also include negative control water samples taken alongside experiment samples (n=18) and blank samples taken as controls during the DNA extraction process (n=2).", "distribution": [{"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P13QHKMG", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}, {"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.6881d3acd4be0232bdf2ae29.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_6881d3acd4be0232bdf2ae29", "keyword": ["Hawaii", "Hawaii Volcanoes National Park", "USGS:6881d3acd4be0232bdf2ae29", "biota", "environment", "environmental DNA", "field inventory and monitoring", "health", "mosquito larval development", "mosquito monitoring", "quantitative polymerase chain reaction", "water sampling"], "modified": "2026-04-08T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-155.250614, 19.418334, -155.246429, 19.421187", "theme": ["geospatial"], "title": "Culex quinquefasciatus Life Cycle, Development Stages Lab Experiment, Water Filter Sampling Data and Quantitative PCR Results, 2024-2025"}