Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "009:25" ] |
| contactPoint |
{ "fn": "NIH", "@type": "vcard:Contact", "hasEmail": "mailto:info@nih.gov" } |
| description | Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Official Government Data Source", "mediaType": "text/html", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126239/" } ] |
| identifier | https://healthdata.gov/api/views/f4wx-3iqh |
| issued | 2025-07-14 |
| keyword |
[ "gene-expression", "housekeeping-genes", "nih", "normalization-methods", "rt-pcr" ] |
| landingPage | https://healthdata.gov/d/f4wx-3iqh |
| modified | 2025-09-06 |
| programCode |
[ "009:033" ] |
| publisher |
{ "name": "National Institutes of Health", "@type": "org:Organization" } |
| theme |
[ "NIH" ] |
| title | Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes |
