Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"009:25"
]
|
| contactPoint |
{
"fn": "NIH",
"@type": "vcard:Contact",
"hasEmail": "mailto:info@nih.gov"
}
|
| description | Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Official Government Data Source",
"mediaType": "text/html",
"description": "Visit the original government dataset for complete information, documentation, and data access.",
"downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126239/"
}
]
|
| identifier | https://healthdata.gov/api/views/f4wx-3iqh |
| issued | 2025-07-14 |
| keyword |
[
"gene-expression",
"housekeeping-genes",
"nih",
"normalization-methods",
"rt-pcr"
]
|
| landingPage | https://healthdata.gov/d/f4wx-3iqh |
| modified | 2025-09-06 |
| programCode |
[
"009:033"
]
|
| publisher |
{
"name": "National Institutes of Health",
"@type": "org:Organization"
}
|
| theme |
[
"NIH"
]
|
| title | Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes |
