5 datasets found
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Federal
Modulation of L-type Ca
U.S. Department of Health & Human Services —
Background The multisubunit (α1S,α2-δ, β1a and γ1) skeletal muscle dihydropyridine receptor (DHPR) transduces membrane depolarization into release of Ca2+ from the... -
Federal
Specificity of DNA triple helix formation analyzed by a FRET assay
U.S. Department of Health & Human Services —
Background A third DNA strand can bind into the major groove of a homopurine duplex DNA to form a DNA triple helix. Sequence specific triplex formation can be applied... -
Federal
An efficient method to successively introduce transgenes into a given genomic locus in the mouse
U.S. Department of Health & Human Services —
Background Expression of transgenes in mice requires transcriptional regulatory elements that direct expression in a chosen cell type. Unfortunately, the availability... -
Federal
A sensitive and rapid assay for homologous recombination in mosquito cells: impact of vector topology and implications for gene targeting
U.S. Department of Health & Human Services —
Background Recent progress in insect transgenesis has been dramatic but existing transposon-based approaches are constrained by position effects and potential... -
Federal
Gene targeting in mosquito cells: a demonstration of 'knockout' technology in extrachromosomal gene arrays
U.S. Department of Health & Human Services —
Background Gene targeting would offer a number of advantages over current transposon-based strategies for insect transformation. These include freedom from both...
5 datasets found