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Xenopus metamorphosis after neonicotinoid exposure

Metadata Updated: October 29, 2023

Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate treatment group (total n=12 for each treatment) were vivisected and liver, brain, and somatic tissue frozen in RNA/DNA shield for qPCR analyses for another study). On Day 44, tadpoles (n=5) which had reached NF stage 57 were transferred to other vivaria and remained in their original treatment solution through Day 76; these animals were monitored until metamorphosis.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023
Publisher U.S. Geological Survey
Maintainer
@Id http://datainventory.doi.gov/id/dataset/478d2523b907ed66f68b2624cec92a55
Identifier USGS:5ac7c121e4b0e2c2dd110a09
Data Last Modified 20210723
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://datainventory.doi.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 146ebdab-d715-4ab7-8ac2-1db51a01e605
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -94.04,28.93,-88.8,33.02
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash a40c04ea43b88922d6f0f536f93593e504280533e7784dceb8bb0c94064f05b3
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -94.04, 28.93, -94.04, 33.02, -88.8, 33.02, -88.8, 28.93, -94.04, 28.93}

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