The Wyoming statewide time-stamped oil and gas wells shapefile was developed to provide a historical perspective of drilling activity for the Wyoming Landscape Conservation Initiative (WLCI). This product approximates start and stop dates for each well in Wyoming based on database attributes. These data originated from the Wyoming Oil and Gas Conservation Commission (WOGCC). Data represent 120 years of oil and gas drilling (1900 to 2020) and will facilitate a landscape-level approach to integrated science-based assessments, resource management, and land-use decision making. Oil and gas data from WOGCC were used in a dataset previously published by Biewick in 2010 (https://pubs.usgs.gov/ds/625/). This product expands the timeframe of drilling acitivity to 2020, compared to the earlier publication end date of 2010. We extended start and stop dates through 2020 and used a different process to extract start and stop information (detailed in the Process Description portion of Data Quality Information). We did not have access to last completion date (date of the last completion report filing) and did not include production information.