In 2017, the U.S. Fish and Wildlife Service, U.S. Geological Survey, and U.S. Army Corps of Engineers undertook a large-scale interagency field study to determine the influence of commercial barge vessels on the efficacy of the Electric Dispersal Barrier System (EDBS) in the Chicago Sanitary and Ship Canal (CSSC) in preventing fish passage. This study included a series of trials in which a tow, consisting of a tug vessel and fully-loaded barges, transited the EDBS in both upstream-bound (n = 65) and downstream-bound (n = 66) directions. The configuration of the barges for each run is provided in 2017_Run_Information.csv. A 3000 kHz SonTek Argonaut SW Acoustic Doppler Velocity Meter (ADVM), was mounted on the west canal wall at a depth of approximately 4.5 feet (1.7 meters), as measured on July 31, 2017 at 10:00 am CDT. The approximate elevation of the ADVM was 572.21 feet (NAVD88). The wall mounted instrument was located at (41.6423629, -88.060329). The provisional water surface elevation at the USGS streamflow gaging station on the Chicago Sanitary and Ship Canal ranged from 575.23 feet to 577.28 feet (NAVD88) over the study period (August 1 to August 11, 2017). The ADVM measured horizontal profiles of streamwise and cross-stream components of velocity between the edge of tow transiting the EDBS and the West canal wall for each trial. The ADVM reported velocities at 10 bins spaced by 0.5 meters between 0.57 and 6.07 meters from the West canal wall. All velocity measurements represent an average of 10 pings, recorded every 10 seconds during measurement, and are included in this data release as Comma Separated Value (CSV) files. All time data is given in Central Daylight Time (CDT).