The Old Erie Canal has undergone sedimentation and aquatic growth that have restricted flow and diminished the aesthetic quality of the canal during the nearly 200 years since its construction. During 2018–2019, the U.S. Geological Survey (USGS) in cooperation with the Madison County Planning Department and the New York State Canal Corporation conducted a study of the Old Erie Canal between the Town of DeWitt, New York, and its junction with the current Erie Canal of the New York State Canal System near Rome, N.Y. The study comprised bathymetric, velocity, and water-quality surveys and documentation of the canal infrastructure. The USGS established benchmarks and staff gages along the 30.8 miles of the canal study area to reference the water-surface level in the canal to the North American Vertical Datum of 1988 (NAVD 88). Water-quality data (dissolved oxygen, water temperature, specific conductance, pH, and turbidity) were collected concurrently with the bathymetric survey (spring 2018) to characterize changes in water quality along the length of the canal. The canal infrastructure was documented to provide a baseline assessment. This dataset contains a shapefile representing water quality observations made with a multi-parameter sonde during the 2018 survey, and includes measurements of water temperature, turbidity, pH, dissolved oxygen, and specific conductance along transects of Old Erie Canal.