This data release includes metrics from the Regional Stream Quality Assessment (RSQA) from the Southeast Region for habitat stressors related to water-quality and habitat substrate. The goals of RSQA are to characterize multiple water-quality factors that are stressors to aquatic life ‐ contaminants, nutrients, sediment, and streamflow alteration – and to develop a better understanding of the relation of these stressors to ecological conditions in streams throughout the region. In order to characterize water-quality variables and stream-habitat measurements as an aggregation of multiple measurements over a sampling period, and in support of ecological stressor modelling, metrics (summary statistics or indices) were computed from individual results by site using consistent methods over a consistent time frame. Water-quality metrics are based on discrete samples as well as long-term deployed passive samplers.