Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content

Virus and bacteria concentration from wastewater

Metadata Updated: March 16, 2024

Numerous studies have shown that adsorption-extraction (AE) method holds promise, yet several uncertainties remain regarding the optimal AE workflow. Several procedural components may influence the recovered concentrations of target nucleic acid, including membrane types, homogenization instruments, speed and duration, and lysis buffer. In this study, 42 different AE workflows that varied these components were compared to determine the optimal workflow by quantifying endogenous SARS-CoV-2, human adenovirus 40/41 (HAdV 40/41), and a bacterial marker gene of fecal contamination (Bacteroides HF183). Our findings suggest that the workflow chosen had a significant impact on SARS-CoV-2 concentrations, whereas it had minimal impact on HF183 and no effect on HAdV 40/41 concentrations. This dataset is not publicly accessible because: data are not owned by the EPA. It can be accessed through the following means: Contact the corresponding author, Warish Ahmed (Warish.Ahmed@csiro.au). Format: data are not owned by the EPA.

This dataset is associated with the following publication: Akter, J., W.J.M. Smith, Y. Liu, S.L. Simpson, P. Thai, A. Korajkic, and W. Ahmed. Comparison of adsorption-extraction (AE) workflows for improved measurements of viral and bacterial nucleic acid in untreated wastewater. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 908: 167966, (2024).

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

Downloads & Resources

No file downloads have been provided. The publisher may provide downloads in the future or they may be available from their other links.

References

https://doi.org/10.1016/j.scitotenv.2023.167966
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927021

Dates

Metadata Created Date March 16, 2024
Metadata Updated Date March 16, 2024

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date March 16, 2024
Metadata Updated Date March 16, 2024
Publisher U.S. EPA Office of Research and Development (ORD)
Maintainer
Identifier https://doi.org/10.23719/1530413
Data Last Modified 2023-10-18
Public Access Level public
Bureau Code 020:00
Schema Version https://project-open-data.cio.gov/v1.1/schema
Harvest Object Id f23d4da9-28cd-4dbb-8129-3bdb2a24960b
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
License https://pasteur.epa.gov/license/sciencehub-license-non-epa-generated.html
Program Code 020:000
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents https://doi.org/10.1016/j.scitotenv.2023.167966, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927021
Source Datajson Identifier True
Source Hash e4b98db925e35d3e3e788317179e06728671d3dbefdc1eeb544d0392b0df9588
Source Schema Version 1.1

Didn't find what you're looking for? Suggest a dataset here.