Numerous studies have shown that adsorption-extraction (AE) method holds promise, yet several uncertainties remain regarding the optimal AE workflow. Several procedural components may influence the recovered concentrations of target nucleic acid, including membrane types, homogenization instruments, speed and duration, and lysis buffer. In this study, 42 different AE workflows that varied these components were compared to determine the optimal workflow by quantifying endogenous SARS-CoV-2, human adenovirus 40/41 (HAdV 40/41), and a bacterial marker gene of fecal contamination (Bacteroides HF183). Our findings suggest that the workflow chosen had a significant impact on SARS-CoV-2 concentrations, whereas it had minimal impact on HF183 and no effect on HAdV 40/41 concentrations. This dataset is not publicly accessible because: data are not owned by the EPA. It can be accessed through the following means: Contact the corresponding author, Warish Ahmed (Warish.Ahmed@csiro.au). Format: data are not owned by the EPA.
This dataset is associated with the following publication:
Akter, J., W.J.M. Smith, Y. Liu, S.L. Simpson, P. Thai, A. Korajkic, and W. Ahmed. Comparison of adsorption-extraction (AE) workflows for improved measurements of viral and bacterial nucleic acid in untreated wastewater. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 908: 167966, (2024).