Vegetative buffer strips have been demonstrated to effectively reduce loads of sediment, nutrients, and herbicides in surface runoff, but their effectiveness for reducing veterinary antibiotic loads in runoff has not been well documented. Surface runoff simulation where performed to determine the effectiveness of Vegetative buffer strips vegetation and width on surface runoff loads of the veterinary antibiotics sulfamethazine and lincomycin. The three datasets in this release document 1. physical and chemical soil properties for soil located on experimental vegetated buffer strip plots prior to surface runoff simulations; 2. Rainfall simulator performance data and antecedent soil water content data for all vegetated buffer strip plots during the surface runoff simulations; and 3. Surface water hydrology data collected for determining veterinary antibiotic residue load concentrations and suspended sediment load concentrations in all vegetated buffer strip plots during rainfall simulations.