This release consists of vegetation data collected across an environmental gradient at Ten Thousand Islands National Wildlife Refuge (TTI NWR) near Naples, Florida, USA. The refuge is within the impact area of the Picayune Strand Restoration Project (PSRP), a large-scale hydrologic restoration project in southwest Florida that was authorized by the U.S. Congress in 2007. Vegetation transects were located in the following wetland types on the refuge: brackish marsh, salt marsh, and transition marsh. Data from vegetation transects established in nearby areas outside of the PSRP impact area, in Fakahatchee Strand Preserve State Park (FSPSP) and Big Cypress National Preserve (BICY), are also included. The study spanned nine years, from 2007 to 2016. A collection of photographs taken over time at each vegetation transect is included with this Data Release. Information provided with each photograph includes the transect name and the date the image was obtained. Five separate directories of photographs are organized by identified wetland types and are designated: Control Saline Marsh sites, Ten Thousand Islands Brackish Marsh sites, Ten Thousand Islands Salt Marsh sites, Ten Thousand Islands Transition Marsh sites, and Ten Thousand Islands Burn Marsh sites.