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Validation of a Novel Cell Culture Exposure System (CCES) for Studying the Toxicity of Volatile Chemicals at the Air-Liquid Interface

Metadata Updated: November 12, 2020

We developed a cell culture exposure system (CCES) to expose cells at the air-liquid interface (ALI) to volatile chemicals. We characterized the CCES by exposing indigo dye-impregnated filters inside each culture well to 125 ppb ozone (O3) for 1 h at flow rates of 5 and 25 mL/min/well; the reaction of O3 with an indigo dye produces a fluorescence product. We observed a 5-fold increase in fluorescence at 25 mL/min/well, suggesting higher flows were more effective. We then exposed primary human bronchial lung epithelial cells (HBECs) to 0.3 ppm acrolein for 2 h at 3, 5 and 25 mL/min/well and compared our results against well-established Human Studies Facility in vitro exposure chambers (HSF Chambers) at the U.S. EPA. We measured lactate dehydrogenase (LDH) release, and transcript changes of heme oxygenase-1 (HMOX1) and interleukin-8 (IL8) at 0, 1, and 24 h post-exposure. Comparing responses from the HSF Chamber to the CCES, differences were only observed at 1 h post-exposure for HMOX1. Here, the HSF Chamber produced a ~6-fold increase in HMOX1 while the CCES at 3 and 5 mL/min/well produced a ~1.7-fold increase. Operating the CCES at 25 mL/min/well produced a ~4.5-fold increase; slightly lower than the HSF Chamber. Our results suggest that higher flow rates in the CCES were more effective at delivering the gas to the cells, and this was further validated by our comparison against a well-established in vitro exposure system. Further testing is required to explore the sensitivity of the CCES with other chemicals.

This dataset is associated with the following publication: Zavala-Mendez, J., A. Ledbetter, D.S. Morgan, L. Dailey, E. Puckett, S. McCullough, and M. Higuchi. A New Cell Culture Exposure System (CCES) for Studying the Toxicity of Volatile Chemicals at the Air-Liquid Interface. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 30(4): 169-177, (2018).

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

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References

https://doi.org/10.1080/08958378.2018.1483983

Dates

Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Publisher U.S. EPA Office of Research and Development (ORD)
Maintainer
Identifier https://doi.org/10.23719/1394117
Data Last Modified 2017-08-09
Public Access Level public
Bureau Code 020:00
Schema Version https://project-open-data.cio.gov/v1.1/schema
Harvest Object Id ecde981f-aa79-4798-becc-fc31044e374d
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
License https://pasteur.epa.gov/license/sciencehub-license.html
Program Code 020:000
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents https://doi.org/10.1080/08958378.2018.1483983
Source Datajson Identifier True
Source Hash 2003852f81857438c2110aeff10e606921551788
Source Schema Version 1.1

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