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    • Transcriptional profiling of roots and shoots from Brachypodium distachyon seedlings flown on the ISS

    Metadata Updated: August 30, 2025

    Most major cereal grain crops are monocots. Yet, most investigations of plant adaptation to the spaceflight environment have been carried out on the dicotyledonous model plant, Arabidopsis thaliana. It remains unknown whether the conclusions from such studies can be extrapolated to monocotyledonous plants. To fill this knowledge gap, seedlings of three different accessions of Brachypodium distachyon (Bd21, Bd21-3, and Gaz8), a model for temperate grasses including cereal crops, were grown on the International Space Station in the Veggie hardware and on the ground in matched conditions. To synchronize germination, seedlings were grown for 24 hours in red light. Then, seedlings were grown with green/blue/red light using a 24 hour photo period for 4 additional days. At this point, seedlings were harvested and placed in room temperature RNAlater for 24 hours before being moved to -80 C for storage and later dissection into roots and shoots. This dataset features ribodepleted RNA-seq data from the Bd21, Bd21-3 and Gaz8 accessions.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Created Date April 11, 2025
    Metadata Updated Date August 30, 2025

    Metadata Source

    Harvested from NASA Data.json

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date April 11, 2025
    Metadata Updated Date August 30, 2025
    Publisher Open Science Data Repository
    Maintainer
    Identifier 10.26030/2x6b-3v89
    Data Last Modified 2025-08-21
    Category Biological and Physical Sciences
    Public Access Level public
    Bureau Code 026:00
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 3b39fd56-1638-4707-a96d-de3aa47ed300
    Harvest Source Id 58f92550-7a01-4f00-b1b2-8dc953bd598f
    Harvest Source Title NASA Data.json
    Homepage URL https://lsda.jsc.nasa.gov/Mission/miss/1398
    Program Code 026:000
    Source Datajson Identifier True
    Source Hash 35a61b0cc07934b97ecafce93148c10969334e35078748903a2e570d4632affc
    Source Schema Version 1.1

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