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Transcription profiling of Drosophila after exposure to microgravity in the International Space Station and in a microgravity simulator
Larvae-Pupae transition flies (Drosophila) were recovered and transport for 3 days at 12-14C to arrest development until the launch site then exposed to RT (18-20C) for some hours including the launch and trip to the International Space Station then pupae were exposed to microgravity in the ISS for 4 days and a half at 22C. Finally pupae were fixed on acetone and frozen until recovery on Earth. Four groups of samples: 1 ISS (+ground control) as described 2 RPM (microgravity simulator on Earth) as described 3 RPM without constrains (No MAMBA container and only 5 days exposure without cold transport) and 4 centrifuge 10g without constrains control.
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During development the Sku5 mutant roots engage different genes than wild type WS roots either on the ground or in spaceflight.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "026:00" ] |
| contactPoint |
{ "fn": "GeneLab Outreach", "@type": "vcard:Contact", "hasEmail": "mailto:genelab-outreach@lists.nasa.gov" } |
| description | Larvae-Pupae transition flies (Drosophila) were recovered and transport for 3 days at 12-14C to arrest development until the launch site then exposed to RT (18-20C) for some hours including the launch and trip to the International Space Station then pupae were exposed to microgravity in the ISS for 4 days and a half at 22C. Finally pupae were fixed on acetone and frozen until recovery on Earth. Four groups of samples: 1 ISS (+ground control) as described 2 RPM (microgravity simulator on Earth) as described 3 RPM without constrains (No MAMBA container and only 5 days exposure without cold transport) and 4 centrifuge 10g without constrains control. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "During development the Sku5 mutant roots engage different genes than wild type WS roots either on the ground or in spaceflight.", "format": "HTML", "mediaType": "text/html", "description": "GeneLab Study Page", "downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-281" } ] |
| identifier | nasa_genelab_GLDS-36_mqvy-xyjb |
| issued | 2021-05-21 |
| keyword |
[ "affymetrix-protocol-hybridization-unknown", "altered-gravity", "altered-gravity-simulator", "data-transformation", "feature_extraction", "genelab-microarray-data-processing-protocol", "grow", "hybridization", "labeling", "nucleic_acid_extraction", "p-affy-2", "p-affy-6", "p-mtab-3321", "p-mtab-3322", "spaceflight", "treatment" ] |
| landingPage | https://data.nasa.gov/dataset/transcription-profiling-of-drosophila-after-exposure-to-microgravity-in-the-international- |
| license | http://www.usa.gov/publicdomain/label/1.0/ |
| modified | 2025-04-23 |
| programCode |
[ "026:005" ] |
| publisher |
{ "name": "National Aeronautics and Space Administration", "@type": "org:Organization" } |
| theme |
[ "Earth Science" ] |
| title | Transcription profiling of Drosophila after exposure to microgravity in the International Space Station and in a microgravity simulator |
