As part of a cooperative study by the Alabama Department of Transportation (ALDOT) and the U.S. Geological Survey, topographic surveys of stream channel cross-sections, upstream and downstream of selected new culvert installations in the East Gulf Coastal Plain of Alabama, were conducted both before and after culvert construction. This dataset contains raw topographic data used to determine channel measurements for statistical analysis in the associated Scientific Investigations Report, Effects of culvert construction on streams in the East Gulf Coastal Plain of Alabama, 2010-19 (Pugh and Gill, 2021). To measure the effects that culvert construction may have on stream channel beds, banks, and slopes, topographic surveys of 22 stream channel cross-sections, 11 upstream and 11 downstream, of the proposed culvert, were conducted at each study site before culvert construction. The cross-sections were evenly distributed along a stream reach length of approximately 20 times the channel width. These same cross-sections were resurveyed approximately 2 years after culvert construction was completed. Pre- and post-construction channel geometry data are presented in separate Comma-Separated Values (CSV) files. In addition, a plan view of before and after construction data points is included in a Portable Document Format (PDF) file.