This data release presents chemical and biological results from an investigation of the uptake of per- and polyfluoroalkyl substances (PFAS) from groundwater contaminated by fire training activities on Cape Cod, Massachusetts conducted from July to August 2019. An exposure experiment was conducted at an a PFAS contaminated site (FTA-1) near the fire training area (FTA). To assess the tissue-specific uptake characteristics of the mixture of PFAS present in the groundwater from the FTA-1 site, a 21-day mobile laboratory exposure experiment was conducted. Details for the groundwater sampling sites and well construction are presented (Table 1). The PFAS mixture concentrations and composition in groundwater and in fish tissues was evaluated using 2 separate analytical methods (Table 2). PFAS quality assurance data are presented in Table 3. Water and tissue samples were collected on days 1, 7, and 21. Water chemistry results for target PFAS analysis are summarized in Table 4. Water chemistry results for suspect PFAS analysis are summarized in Table 5. Fish morphometric data are summarized in Table 6. Fish tissue PFAS target analysis results are presented in Table 7. Fish tissue PFAS suspect analysis results are presented in Table 8.