The U.S. Geological Survey (USGS) modeled the distribution of terrestrial ecosystems for the contiguous United States using a standardized, deductive approach to associate unique physical environments with ecological systems characterized in NatureServe's Ecological Systems of the United States classification (Comer et al., 2003). This approach was first developed for South America (Sayre et al., 2008) and is now being implemented globally (Sayre et al., 2007). Unique physical environments were delineated from a massive biophysical stratification of the nation into the major structural components of ecosystems: biogeographic regions (Cress et al., 2008c), land surface forms (Cress et al., 2008a), surficial lithology (Cress et al., 2008d), and topographic moisture potential (Cress et al., 2008b). Each of these structural components was mapped for the contiguous United States and then spatially combined to produce ecosystem structural footprints which represented unique abiotic (physical) environments. Among 49,168 unique structural footprint classes, 13,482 classes which met a minimum pixel count threshold (20,000 pixels) were aggregated into 419 NatureServe ecosystems through semi-automated labeling process using rule set formulations for attribution of each ecosystem. UPDATE: A newer terrestrial ecosystems datalayer, "World Terrestrial Ecosystems (WTE) 2020", is now available at: https://doi.org/10.5066/P9DO61LP. This datalayer is a global raster dataset at a 250 m spatial resolution where 431 ecosystem types are identified and mapped. Each ecosystem type is a unique combination of vegetation/land cover, climate region, and landform.