Streamflow wading measurements were made on March 26, 2014 within the Deer Creek watershed in St. Louis County, Missouri, following U.S. Geological Survey methods in Turnipseed and Sauer (2010). This effort occurred during a date and time void of rainfall or snowmelt runoff to properly evaluate a base-flow condition. Measuring locations were chosen based on inflow junctions (for example open channel tributaries or pipe outflows) such that main stem streamflow could be evaluated above and below the inflow. A total of 31 main stem and 25 inflow streamflow measurements were made over 9 miles along the main stem reach of Deer Creek starting at Magna Carta Drive. This data release includes a table of the streamflow measurements in comma separated values (.csv) format and a map of the main stem and inflow junction measurement locations (Figure 1) and graphical representation of the main stem streamflow correlated to distance downstream from Magna Carta Drive (Figure 2).