The U.S. Geological Survey (USGS), in cooperation with the Puerto Rico Environmental Quality Board, has compiled a series of geospatial datasets for Puerto Rico to be implemented into the USGS StreamStats application (https://streamstats.usgs.gov/ss/). These geospatial datasets, along with basin characteristics datasets for Puerto Rico published as a separate USGS data release (https://doi.org/10.5066/P9HK9SSQ), were used to delineate watersheds and develop the peak-flow and low-flow regression equations used by StreamStats. The geospatial dataset described herein are the stream definition rasters with a 150,000 stream cell threshold at a 10-m resolution. The flow accumulation grid is used as input to create the stream grid. This requires a flow accumulation of 150,000 pixels or greater to initiate a stream channel. A value of 1 is assigned for all of the cells equal or greater than the threshold and no data for all other cells. Data are partitioned into four TIFF files, one for each of the four 8-digit Hydrologic Unit Code (HUC) areas for Puerto Rico: 21010002, 21010003, 21010004, and 21010005.