Montgomery County, Maryland Department of Environmental Protection has collected datasets to assess the health of streams since the early 1990s. Datasets include geomorphic stream cross-sectional surveys, fish and benthic macroinvertebrate counts and taxa abundance, and water chemistry data collected at the time of benthic and fish sampling (dissolved oxygen, pH, specific conductance, air temperature, and water temperature). Data span years 1992 to 2020 at five watersheds within the Clarksburg study area. Watersheds include a forested reference site (Soper), an urban site with centralized stormwater management (Crystal Rock), and three treatment watersheds (TR104, TR109, and Cabin Branch) within the Clarksburg Special Protection Area that transitioned from agriculture to suburban development with distributed stormwater management. These data were used to assess the impacts of distributed stormwater management on stream ecosystem function. All datasets were collected by Montgomery County, Maryland Department of Environmental Protection.