S&T Project 19009 Final Report: Evaluating the Sensitivity and Reproducability of Environmental DNA Field Sampling Workflows for Detection of Dreissenid Mussels
There is a need to develop and evaluate eDNA dreissenid mussel field sampling protocols. We compared two common eDNA field sampling methods, sub-surface grab samples and plankton tow samples, in 12 waterbodies with known or potential dreissenid mussel infestations spanning the coterminous United States. To mimic early detection sampling scenarios, we sampled waterbodies or areas within waterbodies that had lower densities of dreissenid mussels or were suspect for dreissenid mussels. The two eDNA sampling methods resulted in similar dreissenid mussel DNA detection patterns. Both methods detected target DNA at the same 7 waterbodies and both methods failed to detect target DNA at the same 5 waterbodies. At one of the lower density sites, more mussel DNA was amplified in plankton tow samples than from grab samples.
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RISE Item Details Page URL for "S&T Project 19009 Final Report: Evaluating the Sensitivity and Reproducability of Environmental DNA Field Sampling Workflows for Detection of Dreissenid Mussels"
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PDF File for "S&T Project 19009 Final Report: Evaluating the Sensitivity and Reproducability of Environmental DNA Field Sampling Workflows for Detection of Dreissenid Mussels"
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[ "010:10" ] |
| contactPoint |
{ "fn": "RISE Team", "@type": "vcard:Contact", "hasEmail": "mailto:data@usbr.gov" } |
| description | There is a need to develop and evaluate eDNA dreissenid mussel field sampling protocols. We compared two common eDNA field sampling methods, sub-surface grab samples and plankton tow samples, in 12 waterbodies with known or potential dreissenid mussel infestations spanning the coterminous United States. To mimic early detection sampling scenarios, we sampled waterbodies or areas within waterbodies that had lower densities of dreissenid mussels or were suspect for dreissenid mussels. The two eDNA sampling methods resulted in similar dreissenid mussel DNA detection patterns. Both methods detected target DNA at the same 7 waterbodies and both methods failed to detect target DNA at the same 5 waterbodies. At one of the lower density sites, more mussel DNA was amplified in plankton tow samples than from grab samples. |
| distribution |
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| identifier | https://datainventory.usbr.gov/rise/item/72318 |
| keyword |
[ "Detection", "Dreissenid Mussels", "Environmental DNA (eDNA)", "Quagga Mussels", "Zebra Mussels" ] |
| landingPage | https://data.usbr.gov/catalog/6376/item/72318 |
| modified | 2022-09-19T14:53:04Z |
| publisher |
{ "name": "Bureau of Reclamation", "@type": "org:Organization" } |
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| title | S&T Project 19009 Final Report: Evaluating the Sensitivity and Reproducability of Environmental DNA Field Sampling Workflows for Detection of Dreissenid Mussels |
