Hydroacoustic (sonar) data were collected for the Mississippi, St. Croix, and Minnesota Rivers for the development of high-resolution bathymetry and sidescan imagery. Small areas containing priority mussel habitat had additional collection efforts to map water velocities and bottom composition. Combining these data in a GIS can provide key components to characterizing physical benthic habitat for native mussels in a riverine environment. These information needs were highly desired by the National Park Service to more accurately assess environmental factors that influence native mussel distribution. The collaborative effort was funded by the Legislative-Citizen Commission on Minnesota Resources (LCCMR) Environment and Natural Resources Trust Fund (ENRTF), to help maintain and enhance Minnesota’s environment and natural resources.