These data were collected using a 1200 kHz TRDI Rio Grande acoustic Doppler current profiler (ADCP) in mode 12 with 25 centimeter bins from a moving boat. The data were georeferenced with a Trimble AG132 differential Global Positioning System (GPS) receiver with submeter accuracy. The data have been depth-averaged over the entire measured portion of the water column, temporally averaged over 5-second intervals to reduce noise, and exported as a .csv file using the Velocity Mapping Toolbox (Parsons and others, 2013). These data were collected in support of the US Army Corps of Engineers Great Lakes and Mississippi River Interbasin Study (GLMRIS), and were concurrent with a dye-tracing study. NOTE: Any data assigned a value of "-9999" are invalid or missing data and should not be used for analysis. Parsons, D. R., Jackson, P. R., Czuba, J. A., Engel, F. L., Rhoads, B. L., Oberg, K. A., Best, J. L., Mueller, D. S., Johnson, K. K. and Riley, J. D. (2013), Velocity Mapping Toolbox (VMT): a processing and visualization suite for moving-vessel ADCP measurements. Earth Surf. Process. Landforms, 38: 1244–1260. doi: 10.1002/esp.3367