Subterranean estuaries extend inland into density-stratified coastal carbonate aquifers that contain a surprising diversity of endemic animals (mostly crustaceans) within a highly oligotrophic environment. How complex ecosystems thrive in this globally-distributed, cryptic habitat (termed anchialine) is poorly understood. The northeastern margin of the Yucatan Peninsula contains over 250 km of mapped, diver-accessible caves passages where previous studies have suggested chemoautotrophic processes are the source of carbon and energy sustaining the anchialine food web. This dataset, collected during four field events during U.S. Geological Survey (USGS) Coastal and Marine Geology Program Field Activities 2015-013-FA and 2016-003-FA in conjunction with Texas A&M University reports physical and chemical properties of the water column from Cenote Bang, a component of the Ox Bel Ha cave network that is located 5 km inland from the coast.