This dataset contains morphometric information from Burmese pythons collected from an invasive population in southern Florida between 1995-2021. Scientists from the U.S. Geological Survey and the National Park Service curated this dataset as a repository for records of Burmese pythons found on or nearby federal lands in southern Florida, including Everglades National Park, Big Cypress National Preserve, Biscayne National Park, and Crocodile Lake National Wildlife Refuge. As such, numerous entities actively or incidentally involved in python research or management activities contributed specimens and/or data to this dataset, including but not limited to the U.S. Geological Survey, National Park Service, U.S. Fish and Wildlife Service, University of Florida, Conservancy of Southwest Florida, Florida Fish and Wildlife Conservation Commission, South Florida Water Management District, volunteers, and members of the public. The dataset includes python identification information, capture information, morphometric data, and necropsy data. The structure of the dataset is such that every row pertains to a single date that data were collected from a single python so that serial captures and morphological data collected from unique individuals can be tracked across time via different rows.