Restoration of the Everglades requires the implementation of many components staged temporally and spatially with results realized on different time and spatial scales. Due to extensive feeding and migratory patterns of manatees, restoration effects on Florida manatees must be modeled monitored over large time and space scales. U.S. Geological Survey efforts have focused on collecting manatee movement data throughout the Ten Thousand Islands (TTI) region, and developing an individual-based model for manatees to illustrate manatee responses to changes in hydrology related to the Picayune Strand Restoration Project (PSRP). This research on manatees is part of the USGS Southeast Ecological Science Center’s (SECSC), Sirernia research project. This report serves as an archive of processed single-beam bathymetry data that were collected in Ten Thousand Islands, Florida in 2009. Geographic information system data products include a XYZ data, bathymetric contours, and USGS quadrangle map. Additional files include formal Federal Geographic Data Committee (FGDC) metadata.