Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content
  • Feedback

    • Shotgun proteomics of thermally challenged Caribbean reef corals (NCEI Accession 0227133)

    Metadata Updated: March 1, 2025

    A proteome profiling approach was taken to characterize the cellular biology of massive corals of the Upper Florida Keys exposed to experimentally elevated temperatures. Specifically, Orbicella faveolata specimens from two inshore (Cheeca Rocks & The Rocks) and one offshore reef (Little Conch) were incubated at either 33°C for 5 days or 32°C days for 31 days (with controls maintained at 30°C), and proteins were extracted from a subset of 16 samples (representing multiple coral genotypes at each temperature x time), separated by liquid chromatography, and sequenced by mass spectrometry (MS). The associated, open-access manuscript (Mayfield et al. 2021) includes all methodological details needed to interpret the proteomic data. However, I have nevertheless reiterated the majority of these details in this document to aid those in navigating the NCEI data submission. Alongside this detailed methodological treatise, I have also uploaded 16 RAW files generated by the MS (Q Exactive™, Thermo-Fisher Scientific [TFS]), 16 MZML (open-access MS mass peaks) files, 16 MZID (open-access MS results) files, two sequence libraries needed to query the proteomic data (fasta files for the coral host & its dinoflagellate [family Symbiodiniaceae] endosymbionts), and a tab-delimited “online supplemental data file” (OSDF) that 1) describes which samples were analyzed; 2) defines MS jargon and explains how to interpret the MS output; and 3) presents all data in a distilled format that is more amenable to analysis by traditional statistical approaches. Please note that there are 32 MZID files, rather than 16, because each of the 16 MZML files was separately queried against each of the two aforementioned fasta sequence libraries.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

    Downloads & Resources

    Dates

    Metadata Date 2025-02-18T14:56:46Z
    Metadata Created Date April 10, 2021
    Metadata Updated Date March 1, 2025
    Reference Date(s) July 30, 2021 (publication)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NOAA/NESDIS/ncei/accessions

    Other Data Resources

    Graphic Preview

    Preview graphic

    Additional Metadata

    Resource Type Dataset
    Metadata Date 2025-02-18T14:56:46Z
    Metadata Created Date April 10, 2021
    Metadata Updated Date March 1, 2025
    Reference Date(s) July 30, 2021 (publication)
    Responsible Party (Point of Contact)
    Contact Email
    Guid gov.noaa.nodc:0227133
    Access Constraints Cite as: Mayfield, Anderson (2021). Shotgun proteomics of thermally challenged Caribbean reef corals (NCEI Accession 0227133). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0227133. Accessed [date]., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
    Bbox East Long -80.50205
    Bbox North Lat 24.95375
    Bbox South Lat 24.89742
    Bbox West Long -80.61573
    Coupled Resource
    Frequency Of Update asNeeded
    Graphic Preview Description Preview graphic
    Graphic Preview File https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0227133
    Graphic Preview Type PNG
    Harvest Object Id d9c0282d-faa5-40df-a079-218494fa5323
    Harvest Source Id c084a438-6f6b-470d-93e0-16aeddb9f513
    Harvest Source Title NOAA/NESDIS/ncei/accessions
    Licence accessLevel: Public
    Lineage
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Polygon", "coordinates": [[[-80.61573, 24.89742], [-80.50205, 24.89742], [-80.50205, 24.95375], [-80.61573, 24.95375], [-80.61573, 24.89742]]]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2017-07-15
    Temporal Extent End 2017-08-31

    Didn't find what you're looking for? Suggest a dataset here.