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    • Segmentation of lipid nanoparticles from cryogenic electron microscopy images

    Metadata Updated: February 23, 2023

    Lipid nanoparticles (LNPs) were prepared as described (https://doi.org/10.1038/s42003-021-02441-2) using the lipids DLin-KC2-DMA, DSPC, cholesterol, and PEG-DMG2000 at mol ratios of 50:10:38.5:1.5. Four sample types were prepared: LNPs in the presence and absence of RNA, and with LNPs ejected into pH 4 and pH 7.4 buffer after microfluidic assembly. To prepare samples for imaging, 3 ?L of LNP formulation was applied to holey carbon grids (Quantifoil, R3.5/1, 200 mesh copper). Grids were then incubated for 30 s at 298 K and 100% humidity before blotting and plunge-freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Grids were imaged at 200 kV using a Talos Arctica system equipped with a Falcon 3EC detector (Thermo Fisher Scientific). A nominal magnification of 45,000x was used, corresponding to images with a pixel count of 4096x4096 and a calibrated pixel spacing of 0.223 nm. Micrographs were collected as dose-fractionated ?movies? at nominal defocus values between -1 and -3 ?m, with 10 s total exposures consisting of 66 frames with a total electron dose of 12,000 electrons per square nanometer. Movies were motion-corrected using MotionCor2 (https://doi.org/10.1038/nmeth.4193), resulting in flattened micrographs suitable for downstream particle segmentation. A total of 38 images were manually segmented into particle and non-particle regions. Segmentation masks and their corresponding images are deposited in this data set.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    References

    https://doi.org/10.1038/s42003-021-02441-2

    Dates

    Metadata Created Date February 23, 2023
    Metadata Updated Date February 23, 2023
    Data Update Frequency irregular

    Metadata Source

    Harvested from NIST

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date February 23, 2023
    Metadata Updated Date February 23, 2023
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-2753
    Data First Published 2023-02-01
    Language en
    Data Last Modified 2022-08-18 00:00:00
    Category Information Technology:Software research, Information Technology:Computational science, Physics:Biological physics, Nanotechnology:Nanobiotechnology, Bioscience:Biomaterials
    Public Access Level public
    Data Update Frequency irregular
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 9721bb83-5590-4d0f-a859-f4f073a9c9aa
    Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
    Harvest Source Title NIST
    Homepage URL https://data.nist.gov/od/id/mds2-2753
    License https://www.nist.gov/open/license
    Program Code 006:045
    Related Documents https://doi.org/10.1038/s42003-021-02441-2
    Source Datajson Identifier True
    Source Hash a4caa258f1d41cb3f0de3beb3af0948b60066cad
    Source Schema Version 1.1

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