From June 2 through 9, 2021, the U.S. Geological Survey (USGS) St. Petersburg Coastal and Marine Science Center (SPCMSC) conducted geophysical surveys to investigate the geologic controls on barrier island evolution and determine Holocene stratigraphy near Santa Rosa Island, Florida (FL). Santa_Rosa_Island_2021_SBES_xyz.zip is a xyz point file dataset of field activity number (FAN) 2021-322-FA single-beam bathymetry (SBB) data collected concurrently with subbottom data to provide a current seafloor elevation reference. The single beam bathymetry data were acquired in the World Geodetic System of 1984, realization G1762 (WGS84[G1762]) for the horizontal (x,y), and WGS84 ellipsoid for the vertical (z). This dataset was then post-processed to obtain Differential Global Positioning System (DGPS) coordinates. The final bathymetry dataset is referenced to WGS84(G1762) Universal Transverse Mercator (UTM) Zone 16 North (N) (x,y) and WGS84 ellipsoid height (z). This dataset was also converted into the North American Datum of 1983 (NAD83) (2011) UTM Zone 16N (x,y) and the North American Vertical Datum of 1988 (NAVD88) (z) with respect to GEOID 12A using using the National Oceanic and Atmospheric Administration’s (NOAA) VDatum version 4.3 transformation software (https://vdatum.noaa.gov/).