Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "019:20" ] |
| contactPoint |
{ "fn": "Yongqin Jiao", "@type": "vcard:Contact", "hasEmail": "mailto:jiao1@llnl.gov" } |
| dataQuality |
true
|
| description | LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Desorption Data.xlsx", "format": "xlsx", "accessURL": "https://gdr.openei.org/files/965/GTO_data_deposit_092017_Desorption.xlsx", "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet", "description": "Excel file detailing results of the bicarbonate and citrate desorption experiments. Plots of results included." } ] |
| DOI | 10.15121/1452718 |
| identifier | https://data.openei.org/submissions/7133 |
| issued | 2017-06-01T06:00:00Z |
| keyword |
[ "REE", "adsorption", "bicarbonate", "brine", "centrifuge", "citrate", "desorption", "energy", "geothermal", "lanthanide binding tag", "lbt", "rare earth", "resource recovery" ] |
| landingPage | https://gdr.openei.org/submissions/965 |
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2018-06-14T16:28:49Z |
| programCode |
[ "019:006" ] |
| projectLead | Holly Thomas |
| projectNumber | LLNL FY17 AOP 2.5.1.12 |
| projectTitle | Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes |
| publisher |
{ "name": "Lawrence Livermore National Laboratory", "@type": "org:Organization" } |
| spatial |
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|
| title | Rare Earth Desorption Test with Citrate and Bicarbonate |
