Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay
These data describe layered double hydroxide (LDH) mediated delivery of novel gene silencing agents (GSAs) in leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i>. Double-stranded and hairpin RNAs were designed and synthesized to target paralogous genes coding for phytoene desaturase (PDS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). A leaf cutting bioassay was developed to rapidly screen GSAs for effective gene silencing action. Reduced expression of <i>PDS</i> and <i>EPSPS</i> were then characterized using reverse transcription quantitative PCR (RT-qPCR), the results of which are presented herein. Related sequences describing target genes from experimental plants are available via the National Center for Biotechnology Information's GenBank database (accession IDs: PQ634570, PQ634571, PQ634572, PQ634573).
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "010:12" ] |
| contactPoint |
{ "fn": "McKenzie Smith", "@type": "vcard:Contact", "hasEmail": "mailto:mksmith@usgs.gov" } |
| description | These data describe layered double hydroxide (LDH) mediated delivery of novel gene silencing agents (GSAs) in leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i>. Double-stranded and hairpin RNAs were designed and synthesized to target paralogous genes coding for phytoene desaturase (PDS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). A leaf cutting bioassay was developed to rapidly screen GSAs for effective gene silencing action. Reduced expression of <i>PDS</i> and <i>EPSPS</i> were then characterized using reverse transcription quantitative PCR (RT-qPCR), the results of which are presented herein. Related sequences describing target genes from experimental plants are available via the National Center for Biotechnology Information's GenBank database (accession IDs: PQ634570, PQ634571, PQ634572, PQ634573). |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Digital Data", "format": "XML", "accessURL": "https://doi.org/10.5066/P1UMSYQY", "mediaType": "application/http", "description": "Landing page for access to the data" }, { "@type": "dcat:Distribution", "title": "Original Metadata", "format": "XML", "mediaType": "text/xml", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.673e4017d34e6b795de6c6e8.xml" } ] |
| identifier | http://datainventory.doi.gov/id/dataset/USGS_673e4017d34e6b795de6c6e8 |
| keyword |
[ "Great Lakes", "North America", "Phragmites australis", "RNA interference", "USGS:673e4017d34e6b795de6c6e8", "biota", "gene expression", "gene silencing", "invasive species" ] |
| modified | 2025-04-10T00:00:00Z |
| publisher |
{ "name": "U.S. Geological Survey", "@type": "org:Organization" } |
| spatial | 172.4800, 7.2000, -12.2900, 83.6200 |
| theme |
[ "geospatial" ] |
| title | Quantitative polymerase chain reaction data describing target gene silencing in the invasive common reed Phragmites australis subsp. australis using a leaf cutting bioassay |
