Quantitative polymerase chain reaction and confocal microscopy data describing cell penetrating peptide-mediated delivery of gene silencing agents to the invasive common reed Phragmites australis
These data describe delivery of novel gene silencing agents (GSAs) to leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i> using cell penetrating peptides (CPPs). These CPPs were tested for their ability to infiltrate <i>P. australis</i> leaf tissue using confocal microscopy. In addition, a series of novel gene silencing agents targeting a putative phytoene desaturase (<i>PDS</i>) gene were developed including twelve antisense oligonucleotides (ASOs), five artificial microRNAs (amiRNAs), and amiRNA-expressing plasmids and a cassette. GSAs were applied to living <i>P. australis</i> plants in conjunction with cell penetrating peptides and an infiltration medium at multiple charge ratios and application frequencies. Reduced expression of <i>PDS</i> was then characterized using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Confocal microscopy and qPCR results are available herein.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "010:12" ] |
| contactPoint |
{ "fn": "McKenzie Smith", "@type": "vcard:Contact", "hasEmail": "mailto:mksmith@usgs.gov" } |
| description | These data describe delivery of novel gene silencing agents (GSAs) to leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i> using cell penetrating peptides (CPPs). These CPPs were tested for their ability to infiltrate <i>P. australis</i> leaf tissue using confocal microscopy. In addition, a series of novel gene silencing agents targeting a putative phytoene desaturase (<i>PDS</i>) gene were developed including twelve antisense oligonucleotides (ASOs), five artificial microRNAs (amiRNAs), and amiRNA-expressing plasmids and a cassette. GSAs were applied to living <i>P. australis</i> plants in conjunction with cell penetrating peptides and an infiltration medium at multiple charge ratios and application frequencies. Reduced expression of <i>PDS</i> was then characterized using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Confocal microscopy and qPCR results are available herein. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Digital Data", "format": "XML", "accessURL": "https://doi.org/10.5066/P13ETPDI", "mediaType": "application/http", "description": "Landing page for access to the data" }, { "@type": "dcat:Distribution", "title": "Original Metadata", "format": "XML", "mediaType": "text/xml", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.66b78f5dd34eebcf8bb3da9f.xml" } ] |
| identifier | http://datainventory.doi.gov/id/dataset/USGS_66b78f5dd34eebcf8bb3da9f |
| keyword |
[ "Phragmites australis", "RNA interference", "USGS:66b78f5dd34eebcf8bb3da9f", "farming", "gene expression", "gene silencing", "invasive species", "molecular biology" ] |
| modified | 2025-01-28T00:00:00Z |
| publisher |
{ "name": "U.S. Geological Survey", "@type": "org:Organization" } |
| spatial | -90.87453, 32.29091, -90.85238, 32.31296 |
| theme |
[ "geospatial" ] |
| title | Quantitative polymerase chain reaction and confocal microscopy data describing cell penetrating peptide-mediated delivery of gene silencing agents to the invasive common reed Phragmites australis |
