Quantitative PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples
A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Adam J Sepulveda",
"@type": "vcard:Contact",
"hasEmail": "mailto:asepulveda@usgs.gov"
}
|
| description | A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Digital Data",
"format": "XML",
"accessURL": "https://doi.org/10.5066/F7C53JDW",
"mediaType": "application/http",
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{
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"title": "Original Metadata",
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_594c30c8e4b062508e385643 |
| keyword |
[
"East Gallatin River",
"Montana",
"Sypes Canyon Creek",
"Tetracapsuloides bryosalmonae",
"USGS:594c30c8e4b062508e385643",
"Yellowstone River",
"biota",
"environmental DNA",
"molecular biology",
"myxozoan",
"parasites",
"qPCR"
]
|
| modified | 2020-08-26T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -124.01367187500001, 38.58252615935333, -103.27148437447, 49.238835165594 |
| theme |
[
"geospatial"
]
|
| title | Quantitative PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples |
