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Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS

Metadata Updated: July 6, 2024

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates to protein with which to normalize the relative luminescence units of the cell lysates.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Maintainer
@Id http://datainventory.doi.gov/id/dataset/45ec8b88ee0e8e59feb474fde85f5f17
Identifier USGS:63860636d34ed907bf779d6d
Data Last Modified 20221213
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://datainventory.doi.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id efa58c4e-2f77-4493-9593-37043efe0b21
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -92.284312,38.910784,-92.27445,38.912612
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash 8dd9758588bf0472b5efd12b5cf4aba6a3c03e3df4bb7a51ac5a514238111f5d
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -92.284312, 38.910784, -92.284312, 38.912612, -92.27445, 38.912612, -92.27445, 38.910784, -92.284312, 38.910784}

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