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Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, primer specificity, 2019-2022

Published by U.S. Geological Survey | Department of the Interior | Catalog Last Checked: May 05, 2026 at 10:08 PM | Dataset Last Updated: January 04, 2024 at 12:00 AM
This data release documents specificity of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia, western mosquito fish and guppies. These assays provide new tools for resource managers to monitor effectiveness of management efforts to remove invasive fish from anchialine pools in Hawaii and to also survey pools for presence and absence of invasive fish. The lab work was conducted during 2019-2022.

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