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Pseudogymnoascus destructans survival at elevated temperatures – Artificial media count data

Metadata Updated: October 29, 2023

The survival of Pseudogymnoascus destructans (Pd) was evaluated at temperatures outside of its thermal range of growth on three different artificial growth media; Sabouraud dextrose agar (SD), brain-heart infusion agar (BHI), and brain-heart infusion agar supplemented with 10% sheep red blood cells (BHI+B). Pd was harvested from starting cultures grown of MEA agar at 7˚C for 60 days. Harvested conidia were diluted in Phosphate Buffered Saline + Tween20 and spread onto plates of a given medium. Plate were then incubated at either 24, 30 or 37˚C. Plates were incubated for 1, 5, 9, 15, 30, 60, 90, 120, or 150 days before being transferred to a 7˚C incubator for 50 days. Colony forming units (CFUs) of Pd were then enumerated, resulting in a time series of Pd survival on a given medium at a given temperature. As each medium was inoculated from a different starting culture of Pd, a control group for each medium was created by inoculating plates as above and then immediate incubation at 7˚C for 50 days. The number of CFUs on the control plates was used as a statistical offset factor which allowed for the fair comparison of Pd survival between different media. The number of conidia initially plated onto each plate varied between 100, 1000 , and 10,000, depending on the temperature-medium treatment combination. In order to ensure robust statistical analysis, all data was rescaled by an appropriate correction factor which is also presented in the datafile.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date October 29, 2023
Publisher U.S. Geological Survey
Identifier USGS:5d14e267e4b0941bde5b749c
Data Last Modified 20200821
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id e0199542-d9de-4584-8e71-985495ecfb12
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -89.485681,43.048161,-89.483278,43.050106
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash 61e3e36b7051cd7fe5cad5703a9d210bf80f1af5b5064bdec4718093b2cedb35
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -89.485681, 43.048161, -89.485681, 43.050106, -89.483278, 43.050106, -89.483278, 43.048161, -89.485681, 43.048161}

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