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Pseudogymnoascus destructans survival at elevated temperatures – Artificial media count data

Metadata Updated: October 1, 2025

The survival of Pseudogymnoascus destructans (Pd) was evaluated at temperatures outside of its thermal range of growth on three different artificial growth media; Sabouraud dextrose agar (SD), brain-heart infusion agar (BHI), and brain-heart infusion agar supplemented with 10% sheep red blood cells (BHI+B). Pd was harvested from starting cultures grown of MEA agar at 7˚C for 60 days. Harvested conidia were diluted in Phosphate Buffered Saline + Tween20 and spread onto plates of a given medium. Plate were then incubated at either 24, 30 or 37˚C. Plates were incubated for 1, 5, 9, 15, 30, 60, 90, 120, or 150 days before being transferred to a 7˚C incubator for 50 days. Colony forming units (CFUs) of Pd were then enumerated, resulting in a time series of Pd survival on a given medium at a given temperature. As each medium was inoculated from a different starting culture of Pd, a control group for each medium was created by inoculating plates as above and then immediate incubation at 7˚C for 50 days. The number of CFUs on the control plates was used as a statistical offset factor which allowed for the fair comparison of Pd survival between different media. The number of conidia initially plated onto each plate varied between 100, 1000 , and 10,000, depending on the temperature-medium treatment combination. In order to ensure robust statistical analysis, all data was rescaled by an appropriate correction factor which is also presented in the datafile.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Created Date September 13, 2025
Metadata Updated Date October 1, 2025

Metadata Source

Harvested from DOI USGS DCAT-US

Additional Metadata

Resource Type Dataset
Metadata Created Date September 13, 2025
Metadata Updated Date October 1, 2025
Publisher U.S. Geological Survey
Maintainer
Identifier http://datainventory.doi.gov/id/dataset/usgs-5d14e267e4b0941bde5b749c
Data Last Modified 2020-08-21T00:00:00Z
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://ddi.doi.gov/usgs-data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id 8bbb40fa-566d-4b5e-b31d-66be9cb0ccb8
Harvest Source Id 2b80d118-ab3a-48ba-bd93-996bbacefac2
Harvest Source Title DOI USGS DCAT-US
Metadata Type geospatial
Old Spatial -89.485681, 43.048161, -89.483278, 43.050106
Source Datajson Identifier True
Source Hash 4181cb349a149d44b96fb68bcf0426e1217523c3a6c8d8509d6f49682c17192e
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -89.485681, 43.048161, -89.485681, 43.050106, -89.483278, 43.050106, -89.483278, 43.048161, -89.485681, 43.048161}

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