Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli
Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2012 and 2013.
Find Related Datasets
Search by Tags
Click any tag below to search for similar datasets
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Cathy A Richter",
"@type": "vcard:Contact",
"hasEmail": "mailto:crichter@usgs.gov"
}
|
| description | Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2012 and 2013. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Digital Data",
"format": "XML",
"accessURL": "https://doi.org/10.5066/P9DGI5F5",
"mediaType": "application/http",
"description": "Landing page for access to the data"
},
{
"@type": "dcat:Distribution",
"title": "Original Metadata",
"format": "XML",
"mediaType": "text/xml",
"description": "The metadata original format",
"downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.62ebfb24d34eacf539724d5e.xml"
}
]
|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_62ebfb24d34eacf539724d5e |
| keyword |
[
"USGS:62ebfb24d34eacf539724d5e",
"alewife",
"biota",
"fish",
"polyacrylamide gel electrophoresis",
"thiaminase",
"thiamine deficiency complex (TDC)",
"zebrafish"
]
|
| modified | 2023-01-17T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -92.284300, 38.910784, -92.274450, 38.912612 |
| theme |
[
"geospatial"
]
|
| title | Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli |
