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Plasma Standards

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Catalog Last Checked: April 21, 2026 at 07:57 PM | Dataset Last Updated: September 22, 2014
Digital droplet PCR fluorsence amplitude values from which plasmid copy concentrations of DNA standards were determined as described in D-EMMD-MEB-018-SOP-01and Journal article. Summary of stability testing results also provided. This dataset is associated with the following publication: Sivaganesan, M., M. Varma, S. Siefring, and R. Haugland. Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 152: 135-142, (2018).

Resources

8 resources available

  • Batch2_Data_Dictionary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_Tube1-5_Data_Dictionary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_Tube1_plasmid_amplitudes_summary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_Tube2_plasmid_amplitudes_summary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_Tube3_plasmid_amplitudes_summary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_Tube4_plasmid_amplitudes_summary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_Tube5_plasmid_amplitudes_summary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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  • Batch2_stability_summary.xlsx

    APPLICATION/VND.OPENXMLFORMATS-OFFICEDOCUMENT.SPREADSHEETML.SHEET
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Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
                                            [
  "020:00"
]
contactPoint 
                                            {
  "fn": "Richard Haugland",
  "hasEmail": "mailto:haugland.rich@epa.gov"
}
description Digital droplet PCR fluorsence amplitude values from which plasmid copy concentrations of DNA standards were determined as described in D-EMMD-MEB-018-SOP-01and Journal article. Summary of stability testing results also provided. This dataset is associated with the following publication: Sivaganesan, M., M. Varma, S. Siefring, and R. Haugland. Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 152: 135-142, (2018).
distribution 
                                            [
  {
    "title": "Batch2_Data_Dictionary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Data_Dictionary.xlsx"
  },
  {
    "title": "Batch2_Tube1-5_Data_Dictionary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube1-5_Data_Dictionary.xlsx"
  },
  {
    "title": "Batch2_Tube1_plasmid_amplitudes_summary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube1_plasmid_amplitudes_summary.xlsx"
  },
  {
    "title": "Batch2_Tube2_plasmid_amplitudes_summary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube2_plasmid_amplitudes_summary.xlsx"
  },
  {
    "title": "Batch2_Tube3_plasmid_amplitudes_summary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube3_plasmid_amplitudes_summary.xlsx"
  },
  {
    "title": "Batch2_Tube4_plasmid_amplitudes_summary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube4_plasmid_amplitudes_summary.xlsx"
  },
  {
    "title": "Batch2_Tube5_plasmid_amplitudes_summary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_Tube5_plasmid_amplitudes_summary.xlsx"
  },
  {
    "title": "Batch2_stability_summary.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1500439/Batch2_stability_summary.xlsx"
  }
]
identifier https://doi.org/10.23719/1500439
keyword 
                                            [
  "digital droplet pcr",
  "dna standards",
  "draft method c",
  "epa methods 1609.1",
  "epa methods 1611.1",
  "qPCR"
]
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2014-09-22
programCode 
                                            [
  "020:096"
]
publisher 
                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
references 
                                            [
  "https://doi.org/10.1016/j.mimet.2018.07.005"
]
rights 
                                            null
title Plasma Standards

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