This dataset contains a combination of physiological and behavioral approaches for characterizing the response of bighead and silver carp to potentially attractive chemicals associated with sex pheromones identified in common carp. The dataset contains eight tables: 1) EOG responses from untreated and masculinized silver carp to prostaglandins, 2) mixture discrimination indices of the prostaglandins tested with the EOG, 3) relative distribution (percentage) of initial behavioral/attractiveness tests of individual PGF’s to silver carp, 4) release rates of prostaglandin mixtures from PGF2α-implanted bigheaded carps, 5) relative distribution (percentage) of masculinized silver carp to PGF2α-implanted carp odors, 6) relative distribution (percentage) of masculinized silver carp to prostaglandins found in PGF2α-implanted carp odors, 7) supplemental data: initial studies of EOG responses from untreated silver carp to prostaglandins, 8) supplemental data: EOG responses from masculinized and untreated silver carp to prostaglandins.