Pd Surveillance Sample Storage-Extraction Experiment Data Release
Storage-Extraction_Experiment.csv: These data include the qPCR results (Ct values and copy numbers) of DNA extracted from swab samples inoculated with Pd conidia (1,000, 10,000, or 100,000 conidia), stored at various temperatures (ambient temperature, 4°C, or -20°C), with or without RNAlater® preservative, for various durations (3 days, 1 month, or 3 months), and extracted with either a Blood and Tissue Kit-based or PrepMan-based protocol. This data set was used to evaluate the effect of both storage condition and DNA extraction method on Pd DNA recovery from swab samples.
LOD_Experiment.csv: These data include qPCR results (Ct values and copy numbers) of DNA extracted from swab samples using either a Blood and Tissue Kit-based or a PrepMan-based protocol following inoculation with a 1:2 dilution series of Pd conidia (4 to 256 conidia). An additional inoculation treatment of 10,000 conidia was also included with this data set. This data set was used to establish and compare the lower limit of detection (in terms of conidia concentration) for the two DNA extraction protocols.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Kyle G George",
"@type": "vcard:Contact",
"hasEmail": "mailto:kgeorge@usgs.gov"
}
|
| description | Storage-Extraction_Experiment.csv: These data include the qPCR results (Ct values and copy numbers) of DNA extracted from swab samples inoculated with Pd conidia (1,000, 10,000, or 100,000 conidia), stored at various temperatures (ambient temperature, 4°C, or -20°C), with or without RNAlater® preservative, for various durations (3 days, 1 month, or 3 months), and extracted with either a Blood and Tissue Kit-based or PrepMan-based protocol. This data set was used to evaluate the effect of both storage condition and DNA extraction method on Pd DNA recovery from swab samples. LOD_Experiment.csv: These data include qPCR results (Ct values and copy numbers) of DNA extracted from swab samples using either a Blood and Tissue Kit-based or a PrepMan-based protocol following inoculation with a 1:2 dilution series of Pd conidia (4 to 256 conidia). An additional inoculation treatment of 10,000 conidia was also included with this data set. This data set was used to establish and compare the lower limit of detection (in terms of conidia concentration) for the two DNA extraction protocols. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Digital Data",
"format": "XML",
"accessURL": "https://doi.org/10.5066/P13RB3ZK",
"mediaType": "application/http",
"description": "Landing page for access to the data"
},
{
"@type": "dcat:Distribution",
"title": "Original Metadata",
"format": "XML",
"mediaType": "text/xml",
"description": "The metadata original format",
"downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.660ede9fd34ededd8cd825ad.xml"
}
]
|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_660ede9fd34ededd8cd825ad |
| keyword |
[
"DNA preservation",
"DNeasy Blood and Tissue Kit",
"PrepMan™",
"RNAlater®",
"USGS:660ede9fd34ededd8cd825ad",
"Ultra Sample Preparation Reagent",
"biota",
"temperature",
"white-nose syndrome"
]
|
| modified | 2025-05-12T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -89.4859, 43.0501, -89.4832, 43.0463 |
| theme |
[
"geospatial"
]
|
| title | Pd Surveillance Sample Storage-Extraction Experiment Data Release |
