Patent AT-E401426-T1: [Translated] METHOD FOR IDENTIFYING BIOLOGICAL SPECIES
The present invention provides a method for identifying species and subspecies in a biological sample through the selective amplification of segments of nucleic acid that code a target region of the cytoplasmatic beta-actin protein, which is present in all the organisms concerned. The method comprises DNA extraction from the sample; amplification of divergent segments of the cytoplasmatic beta-actin gene by PCR or an equivalent technique, using primers of regions with high evolutionary conservation between species and subspecies; and identification of the amplified segment by comparison of its size in base pairs with a pre-established standard of sizes and/or identification of the amplified segment by DNA sequencing and comparison of the resulting sequence with the specific sequence of each species or subspecies present on a computer database.
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Complete Metadata
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|---|---|
| accessLevel | public |
| bureauCode |
[
"009:25"
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| contactPoint |
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"fn": "NIH/NCBI Data Team",
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"hasEmail": "mailto:info@ncbi.nlm.nih.gov"
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|
| description | The present invention provides a method for identifying species and subspecies in a biological sample through the selective amplification of segments of nucleic acid that code a target region of the cytoplasmatic beta-actin protein, which is present in all the organisms concerned. The method comprises DNA extraction from the sample; amplification of divergent segments of the cytoplasmatic beta-actin gene by PCR or an equivalent technique, using primers of regions with high evolutionary conservation between species and subspecies; and identification of the amplified segment by comparison of its size in base pairs with a pre-established standard of sizes and/or identification of the amplified segment by DNA sequencing and comparison of the resulting sequence with the specific sequence of each species or subspecies present on a computer database. |
| distribution |
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"description": "Access the complete Patent AT-E401426-T1: [Translated] METHOD FOR IDENTIFYING BIOLOGICAL SPECIES on the official website.",
"downloadURL": "https://pubchem.ncbi.nlm.nih.gov/patent/AT-E401426-T1"
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|
| identifier | https://healthdata.gov/api/views/xfqj-ha7w |
| issued | 2025-09-05 |
| keyword |
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"chemistry",
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| landingPage | https://healthdata.gov/d/xfqj-ha7w |
| modified | 2025-09-06 |
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| publisher |
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|
| theme |
[
"NIH"
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|
| title | Patent AT-E401426-T1: [Translated] METHOD FOR IDENTIFYING BIOLOGICAL SPECIES |
