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    • Patent AT-E400650-T1: [Translated] METHOD FOR PRODUCING NUCLEIC ACID MOLECULES

    Metadata Updated: September 8, 2025

    Manufacturing a nucleic acid molecule comprising employing at least partially double-stranded oligonucleotide comprising a modification allowing the oligonucleotide to be coupled to a surface, a recognition site for a first type IIS restriction enzyme that cuts outside its recognition site, and a single-stranded overhang, is new. Manufacturing a nucleic acid molecule comprises: (a) providing a first at least partially double-stranded oligonucleotide which has a modification allowing the oligonucleotide to be coupled to a surface, comprises a recognition site for a first type IIS restriction enzyme that cuts outside its recognition site, and comprises a single-stranded overhang; (b) providing a second at least partially double-stranded oligonucleotide, which comprises a recognition site, its part or its complementary sequence, for a second type IIS restriction enzyme which cuts outside its recognition site, and comprises a single-stranded overhang; (c) ligating the first and the second oligonucleotide via their overhangs to generate a first ligation product; (d) immobilizing the first ligation product to the surface via the modification; (e) cutting the immobilized ligation product with the first type IIS restriction enzyme to release an elongated oligonucleotide having an overhang; (f) combining the elongated oligonucleotide with a further at least partially double-stranded oligonucleotide, which has a modification allowing the oligonucleotide to be coupled to a surface, a recognition site for a further type IIS restriction enzyme that cuts outside its recognition site, and a single-stranded overhang, and ligating the elongated second oligonucleotide and the further at least partially double-stranded oligonucleotide via their overhangs to form a further ligation product; (g) immobilizing the further ligation product to a surface via the modification; (h) cutting the further ligation product with the further type IIS restriction enzyme to release an elongated oligonucleotide having an overhang; and (i) optionally, repeating steps (f)-(h).

    Access & Use Information

    Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Created Date September 6, 2025
    Metadata Updated Date September 8, 2025

    Metadata Source

    Harvested from Healthdata.gov

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date September 6, 2025
    Metadata Updated Date September 8, 2025
    Publisher National Center for Biotechnology Information (NCBI)
    Maintainer
    Identifier https://healthdata.gov/api/views/k2ha-i75w
    Data First Published 2025-09-05
    Data Last Modified 2025-09-06
    Category NIH
    Public Access Level public
    Bureau Code 009:25
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Metadata Catalog ID https://healthdata.gov/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 0260e56a-b3be-4e31-9245-ff22c5260146
    Harvest Source Id 651e43b2-321c-4e4c-b86a-835cfc342cb0
    Harvest Source Title Healthdata.gov
    Homepage URL https://healthdata.gov/d/k2ha-i75w
    Program Code 009:066
    Source Datajson Identifier True
    Source Hash 4ff14a501d62b4ba623010b767fcc4f91acd6bf7dbe526396a0b015b97ae265b
    Source Schema Version 1.1

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