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Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Catalog Last Checked: August 03, 2026 at 12:48 AM | Dataset Last Updated: January 01, 2020
Figure 2. Cell non-heme iron concentrations following 4 (A) and 24 (B) hr exposures of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Incubations were without and with 200 μM FAC. Exposures to CB-Air, CB-O3, and SRFA did not impact cell non-heme iron relative to media at either time point. Exposure to FAC increased cell non-heme iron but co-exposure with CB-O3 was associated with significantly increased non-heme iron concentrations at both 4 (A) and 24 (B) hr. Exposures of BEAS-2B cells to FAC increased cell ferritin levels after 24 hr relative to media alone (C). However, co-exposure of CB-O3, and SRFA with FAC was associated with significantly elevated cell ferritin concentrations (C). Two-way ANOVA indicated that both addition of FAC and exposure group significantly impacted cell non-heme iron. Figure 3. Release of IL-8 and IL-6 after 24 hr exposure of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Exposure to CB-O3 and SRFA significantly increased the release of IL-8 and IL-6. FAC treatment significantly diminished this response. This dataset is associated with the following publication: Ghio, A., D.H. Gonzalez, S.E.P. Paulson, J. Soukup, L. Dailey, M. Madden, B. Mahler, S.A. Elmore, M.C. Schladweiler, and U. Kodavanti. Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect. TOXICOLOGIC PATHOLOGY. Society of Toxicology, RESTON, VA, 48(7): 887-898, (2020).

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Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
[
  "020:00"
]
contactPoint 
{
  "fn": "Andrew Ghio",
  "hasEmail": "mailto:ghio.andy@epa.gov"
}
description Figure 2. Cell non-heme iron concentrations following 4 (A) and 24 (B) hr exposures of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Incubations were without and with 200 μM FAC. Exposures to CB-Air, CB-O3, and SRFA did not impact cell non-heme iron relative to media at either time point. Exposure to FAC increased cell non-heme iron but co-exposure with CB-O3 was associated with significantly increased non-heme iron concentrations at both 4 (A) and 24 (B) hr. Exposures of BEAS-2B cells to FAC increased cell ferritin levels after 24 hr relative to media alone (C). However, co-exposure of CB-O3, and SRFA with FAC was associated with significantly elevated cell ferritin concentrations (C). Two-way ANOVA indicated that both addition of FAC and exposure group significantly impacted cell non-heme iron. Figure 3. Release of IL-8 and IL-6 after 24 hr exposure of BEAS-2B cells to media, carbon black treated with air (CB-Air), carbon black treated with ozone (CB-O3), and Suwanee River fulvic acid (SRFA). Exposure to CB-O3 and SRFA significantly increased the release of IL-8 and IL-6. FAC treatment significantly diminished this response. This dataset is associated with the following publication: Ghio, A., D.H. Gonzalez, S.E.P. Paulson, J. Soukup, L. Dailey, M. Madden, B. Mahler, S.A. Elmore, M.C. Schladweiler, and U. Kodavanti. Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect. TOXICOLOGIC PATHOLOGY. Society of Toxicology, RESTON, VA, 48(7): 887-898, (2020).
distribution 
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identifier https://doi.org/10.23719/1519298
keyword 
[
  "Ozone",
  "Rats",
  "air pollution",
  "carbon black",
  "fulvic acid",
  "fulvic acid-like substance",
  "inflammation",
  "iron",
  "lung diseases"
]
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2020-01-01
programCode 
[
  "020:000"
]
publisher 
{
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
references 
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  "https://doi.org/10.1177/0192623320961017",
  "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%202A%20sci%20hub.xlsx",
  "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%202b%20sci%20hub.xlsx",
  "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%202c%20sci%20hub.xlsx",
  "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%203A%20sci%20hub.xlsx",
  "https://pasteur.epa.gov/uploads/10.23719/1519298/documents/ozone%20and%20hulis%20figure%203B%20sci%20hub.xlsx"
]
rights 
null
title Ozone reacts with carbon black to produce a fulvic acid-like substance and increase an inflammatory effect

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