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    • Nutrient data collected from experimental tanks observing the effect of nutrient pollution on Acropora cervicornis disease susceptibility from 2020-09-14 to 2020-11-13 (NCEI Accession 0278916)

    Metadata Updated: November 1, 2023

    This dataset contains the nutrient results for an experiment that assessed the effects of elevated NH4 on corals disease susceptibility. Eleven Acropora cervicornis genotypes were selected to conduct a full factorial tank. Replicate coral fragments (n=24 per genotype) were obtained in July-August 2020 from three South Florida coral nurseries and allowed to recover in tanks in the University of Miami and NOAA’s Experimental Reef Lab (ERL). After recovery from handling, the fragments were evenly allocated to one of eight independent tanks at ERL. For a month and a half, four tanks were maintained under ambient nutrients consistent with nutrient levels present in the seawater from Biscayne Bay, and four tanks were exposed to a 10 μM increase in ammonium concentration (n=12 fragments per genotype per nutrient treatment). To achieve and maintain the nutrient concentrations in the elevated ammonium tanks, a stock solution of NH4Cl was constantly added using peristaltic pumps (~ 10 mL of NH4Cl [2.25 mM] every 15 minutes) to replenish nutrient levels and account for the income of new water in the tanks. After 47 days of nutrient treatments, half of each nutrient cohort was challenged with a disease slurry and half with a placebo slurry, and signs of disease were monitored daily.

    NH4 concentrations were monitored approximately twice a week, and other nutrients (NO2, NO3, PO4, and Si) were estimated for a few time points. Water samples (~ 40 mL) for nutrient analysis were either preserved with 0.5 mL of Chloroform or were frozen until they were processed in the laboratory. The samples were always analyzed for ammonium on a SEAL AA3 Autoanalyzer following EPA methods and NELAC standards. For a few time points phosphate, nitrate+nitrite, nitrite, and silicate were also estimated.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date 2023-06-13T04:00:51Z
    Metadata Created Date June 3, 2023
    Metadata Updated Date November 1, 2023
    Reference Date(s) June 12, 2023 (publication)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NOAA/NESDIS/ncei/accessions

    Other Data Resources

    Graphic Preview

    Preview graphic

    Additional Metadata

    Resource Type Dataset
    Metadata Date 2023-06-13T04:00:51Z
    Metadata Created Date June 3, 2023
    Metadata Updated Date November 1, 2023
    Reference Date(s) June 12, 2023 (publication)
    Responsible Party (Point of Contact)
    Contact Email
    Guid gov.noaa.nodc:0278916
    Access Constraints Cite as: Palacio-Castro, Ana M.; Kroesche, Danielle; Enochs, Ian C.; Kelble, Chris; Rosales, Stephanie (2023). Nutrient data collected from experimental tanks observing the effect of nutrient pollution on Acropora cervicornis disease susceptibility from 2020-09-14 to 2020-11-13 (NCEI Accession 0278916). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0278916. Accessed [date]., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
    Bbox East Long -80.056399
    Bbox North Lat 25.742642
    Bbox South Lat 24.814347
    Bbox West Long -81.241289
    Coupled Resource
    Frequency Of Update asNeeded
    Graphic Preview Description Preview graphic
    Graphic Preview File https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0278916
    Graphic Preview Type PNG
    Harvest Object Id 3c531b28-3f16-403f-82a2-a64da7767901
    Harvest Source Id c084a438-6f6b-470d-93e0-16aeddb9f513
    Harvest Source Title NOAA/NESDIS/ncei/accessions
    Licence accessLevel: Public
    Lineage
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Polygon", "coordinates": [[[-81.241289, 24.814347], [-80.056399, 24.814347], [-80.056399, 25.742642], [-81.241289, 25.742642], [-81.241289, 24.814347]]]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2020-09-14
    Temporal Extent End 2020-11-13

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