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Nuclear inclusion X testing of Pacific Razor clams from select locations in Washington and Alaska
A quantitative PCR (qPCR) assay was developed to detect nuclear inclusion X (NIX) in Pacific razor clams, and assay specificity was confirmed by chromogenic in situ hybridization (CISH). Both tests were applied to evaluate NIX infections in wild Pacific razor clams collected during spring 2019. Consistent with results from earlier histopathological assessments, qPCR and CISH indicated 100% prevalence in razor clams from two Washington beaches and 0% prevalence from two Alaskan beaches.
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "010:12" ] |
| contactPoint |
{ "fn": "Maureen Purcell", "@type": "vcard:Contact", "hasEmail": "mailto:mpurcell@usgs.gov" } |
| description | A quantitative PCR (qPCR) assay was developed to detect nuclear inclusion X (NIX) in Pacific razor clams, and assay specificity was confirmed by chromogenic in situ hybridization (CISH). Both tests were applied to evaluate NIX infections in wild Pacific razor clams collected during spring 2019. Consistent with results from earlier histopathological assessments, qPCR and CISH indicated 100% prevalence in razor clams from two Washington beaches and 0% prevalence from two Alaskan beaches. |
| distribution |
[ { "@type": "dcat:Distribution", "title": "Digital Data", "format": "XML", "accessURL": "https://doi.org/10.5066/P99QQV5B", "mediaType": "application/http", "description": "Landing page for access to the data" }, { "@type": "dcat:Distribution", "title": "Original Metadata", "format": "XML", "mediaType": "text/xml", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5f2d7c4282ceae4cb3c321ea.xml" } ] |
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5f2d7c4282ceae4cb3c321ea |
| keyword |
[ "Alaska Coastal", "USGS:5f2d7c4282ceae4cb3c321ea", "Washington Coastal", "bacteria", "biota", "health", "oceans" ] |
| modified | 2021-10-28T00:00:00Z |
| publisher |
{ "name": "U.S. Geological Survey", "@type": "org:Organization" } |
| spatial | -179.1211, 45.0270, -123.3984, 60.7162 |
| theme |
[ "geospatial" ] |
| title | Nuclear inclusion X testing of Pacific Razor clams from select locations in Washington and Alaska |
