This dataset describes the results of a set of experiments, the goal of which was to evaluate the effects of protease inhibitors on the trout liver S9 substrate depletion assay. The presented data show that addition of phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, substantially increases the working lifetime of the trout S9 assay, resulting in improved detection of low intrinsic clearance rates. These findings substantially increase the utility of the trout S9 assay and may have broad implications for its use to support chemical bioaccumulation assessments.

This dataset is associated with the following publication: Nichols, J., A. Hoffman, J. Swintek, S. Droge, and P. Fitzsimmons. Addition of phenylmethylsulfonyl fluoride (PMSF) substantially increases the working lifetime of the trout liver S9 substrate depletion assay resulting in improved detection of low intrinsic clearance rates. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(1): 148-161, (2021).