The U.S. Geological Survey (USGS) collected hydroacoustic data of the St. Croix River adjacent to the Osceola (WI) boat ramp for hydrographic and benthic mapping prior to the reconstruction project implemented by the National Park Service (NPS). Backscatter is the acoustic reflectivity, which is the measure of energy obtained from the echo intensity, and can provide an indication about the nature of the river bottom and its physical character. Image analysis and classification of backscatter, with the combined datasets of bathymetry (and its derivatives) and ground truthing, can predict surficial substrate, or sediment type. For habitat analysis, these datasets were desired by the NPS to help inform and mitigate potential impacts to mussels or benthic habitat.