Marine geophysical mapping of the Queen Charlotte Fault in the eastern Gulf of Alaska was conducted in 2016 as part of a collaborative effort between the U.S. Geological Survey and the Alaska Department of Fish and Game to understand the morphology and subsurface geology of the entire Queen Charlotte system. The Queen Charlotte fault is the offshore portion of the Queen Charlotte-Fairweather Fault: a major structural feature that extends more than 1,200 kilometers from the Fairweather Range of southern Alaska to northern Vancouver Island, Canada. The data published in this data release were collected along the Queen Charlotte Fault between Cross Sound and Noyes Canyon, offshore southeastern Alaska from May 18 to June 11, 2016. Data were collected aboard the Alaska Department of Fish and Game research vessel Medeia using a Reson SeaBat 7160 multibeam echosounder. This data release contains approximately 453 square kilometers of multibeam bathymetric and backscatter data gridded at 10-meter resolution. Multibeam water column imagery and seismic profile data also collected during this survey are not published in this data release